|
KMID : 1134820110400060818
|
|
Journal of the Korean Society of Food Science and Nutrition
2011 Volume.40 No. 6 p.818 ~ p.823
|
|
|
Effect of Green Tea on Tissue Distribution and Deposition of ¹⁴C-Benzo[a]pyrene in Rats
|
|
Kim Ju-Yeoun
Noh Sang-Kyu
|
|
|
Abstract
|
|
|
|
Recently, we showed that green tea extract (GTE) markedly lowers the intestinal absorption of ¹⁴C-benzo[a]pyrene (¹⁴C-BaP) and enhances its secretion into the biliary route, suggesting a protective role for GTE against body burden. These findings indicate that green tea could be used as an effective dietary means against the toxicity of BaP. The present study, therefore, was designed to investigate if green tea intake could affect the tissue distribution and deposition of ¹⁴C-BaP in rats. Male Sprague-Dawley rats had free access to a nutritionally adequate AIN-93G diet and deionized water. At ∼340 g of weight, the rats were injected intraperitoneally with 27.4 kBq of [4-¹⁴C]-BaP and 5.0 ㎎ of BaP dissolved in 300 ㎕ of olive oil and then assigned randomly to the following two groups: one group (GTE) of rats was fed the AIN-93G diet with GTE via drinking water at approx. 4.7 ㎎ of catechins/d, whereas the other was fed the same diet but without GTE (control). At 4 wk of dietary treatment with GTE, animals were euthanized and heart, liver, brain, spleen, kidney, retroperitoneal fat, testis, and epididymal fat were collected, weighed, and analyzed for tissue ¹⁴C-BaP. Both the control and GTE groups continuously gained weight throughout the study, but there was no significant difference between the groups. No significant differences were observed in the weights of heart, liver, brain, spleen, kidney, retroperitoneal fat, testis, and epididymal fat. However, the radioactivities of ¹⁴C-BaP, expressed in dpm/g, were significantly lower in the heart, liver, brain, spleen, and epididymal fat of rats receiving GTE as compared to their respective controls. These data indicate that green tea intake markedly lowers tissue accumulation of ¹⁴C-BaP. Taken together, these findings suggest that the decreased tissue levels of BaP by GTE intake may be associated with lowered intestinal absorption of BaP and its enhanced secretion into the bile.
|
|
|
KEYWORD
|
|
|
benzo[a]pyrene, distribution, green tea, rats, tissue accumulation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|